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Product Info Summary
| SKU: | M02091 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-NLK Antibody
SKU/Catalog Number
M02091
Size
80 µl
Description
Boster Bio Anti-NLK Antibody (Catalog # M02091). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NLK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02091)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1146CT24.2.1
Isotype
IgG2a
Immunogen
Purified His-tagged NLK protein was used to produced this monoclonal antibody.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02091 is reactive to Nlk in Human
Applications
M02091 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58.313kDa
Background of Nlk
Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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NLK Antibody western blot analysis in SiHa,Hela cell line lysates (35μg/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).
Protein Target Info & Infographic
Gene/Protein Information For Nlk (Source: Uniprot.org, NCBI)
Gene Name
Nlk
Full Name
Serine/threonine-protein kinase NLK
Weight
58.313kDa
Superfamily
protein kinase superfamily
Alternative Names
DKFZp761G1211; EC 2.7.11; EC 2.7.11.24; FLJ21033; LAK1; nemo like kinase; nemo-like kinaseserine/threonine-protein kinase NLK; Protein LAK1 Nlk|AI194375|nemo like kinase|serine/threonine-protein kinase NLK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nlk, check out the Nlk Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nlk: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NLK Antibody (M02091)
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No publications found for M02091
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Customer Reviews
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